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BALB/c mice (female, 6-week-old (Japan SLC, Shizuoka, Japan); n = 6–7; total, 25) were immunized subcutaneously twice at 2 week intervals with 1 µg, 0.5 µg, 0.1 µg, or 0.05 µg doses of the protein in 100 µL PBS.Approximately 3 weeks after the second immunization, mice were anesthetized via intraperitoneal injection of a mixture of 1.0 mg ketamine (Daiichi Sankyo Company, Tokyo, Japan) and 0.02 mg xylazine (Byer Japan, Osaka, Japan) (0.08 ml/10 g of body weight). These mice were then inoculated intranasally with SARS-CoV (106 TCID50 in 30 µl of 2% FBS-MEM).

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血管周围水肿，炎症细胞浸润